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KMID : 0370220190630060374
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Yakhak Hoeji
2019 Volume.63 No. 6 p.374 ~ p.383
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Uncertainty Evaluation for Determination of Methamphetamine and Amphetamine Enantiomers in Urine
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Kim Jin-Young
Sim Yeong-Eun
Ko Beom-Jun
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Abstract
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The objective of this study was to assess the measurement uncertainty for determination of methamphetamineand amphetamine enantiomers in urine sample by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Thedeuterium-labeled compounds were prepared for use as internal standards to quantify the analytes. Urine sample (200 μl)was diluted with pure water before the solid phase extraction (SPE). The sample was extracted and purified using OasisHLB SPE cartridge followed by chromatographic separation on a vancomycin-based chiral column (150 mm×2.1 mm,5 μm). The mass spectrometric analysis were performed in multiple reactions monitoring (MRM) selecting three precursor/fragment ion pairs for each analyte. The potential sources that contribute uncertainty to the final result were evaluated. Themajor contributing factors impacting the combined standard uncertainty were calibration curve and repeatability, while thepreparation of standard solution and sample dilution were minor factors. Relative expanded uncertainty of the measuredanalyte concentration was within 8.5% in a forensic urine sample.
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KEYWORD
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Measurement uncertainty, urine analysis, enantiomer, methamphetamine, amphetamine, LC-MS/MS
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